Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

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• 作者：Nguyen Phuong Thao (1) (2)
  Nguyen Hoai Nam (1)
  Nguyen Xuan Cuong (1)
  Bui Thi Thuy Luyen (1) (2)
  Bui Huu Tai (1)
  Ji Eun Kim (2)
  Seok Bean Song (2)
  Phan Van Kiem (1)
  Chau Van Minh (1)
  Young Ho Kim (2)
  
• 关键词：Soft coral ; Sinularia maxima ; Nuclear factor ; κB ; HepG2 cell ; ICAM ; 1 ; iNOS
• 刊名：Archives of Pharmacal Research
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：37
• 期：6
• 页码：706-712
• 全文大小：
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• 作者单位：Nguyen Phuong Thao (1) (2)
  Nguyen Hoai Nam (1)
  Nguyen Xuan Cuong (1)
  Bui Thi Thuy Luyen (1) (2)
  Bui Huu Tai (1)
  Ji Eun Kim (2)
  Seok Bean Song (2)
  Phan Van Kiem (1)
  Chau Van Minh (1)
  Young Ho Kim (2)
  
  1. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam
  2. College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea
  
• ISSN：1976-3786

文摘

Anti-inflammatory transcriptional effects of nineteen compounds (1-strong class="a-plus-plus">19) from the soft coral Sinularia maxima were evaluated using NF-κB luciferase and reverse transcriptase polymerase chain reaction. Compounds 1, 2, 4, 8, 15, 17, and 18 significantly inhibited TNFα-induced NF-κB transcriptional activity in HepG2 cells in a dose-dependent manner, with IC50 values ranging from 15.81?±?2.29 to 29.10?±?1.54?μM. Furthermore, the transcriptional inhibitory function of these compounds was confirmed by a decrease in intercellular adhesion molecule-1 and inducible nitric oxide synthase gene expression levels in HepG2 cells. These results provide a scientific rationale for the use of the soft coral S. maxima warrant further studies to develop new agents for the prevention and treatment of inflammatory.