Biological treatment of actual petrochemical wastewater using anaerobic/anoxic/oxic process and the microbial diversity analysis
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- 作者：Pengyuan Ding ; Libing Chu ; Jianlong Wang
- 关键词：Hydrolysis ; Acidification ; Anoxic/oxic reactor ; Petrochemical wastewater ; Pyrosequencing
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：100
- 期：23
- 页码：10193-10202
- 全文大小：939 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
- 卷排序：100
文摘
A novel process integrating anaerobic hydrolysis-acidification (HA) and anoxic/oxic (A/O) reactors was developed to treat the actual petrochemical wastewater, which was operated for more than 8 months, the removal efficiency of COD and NH4+-N was monitored, and the microbial community was analyzed. The results showed that the effluent concentrations were maintained at around 99 and 1.3 mg/L, with the removal efficiency of 70.6 and 95.4 %, respectively at a total hydraulic retention time (HRT) of 20 h. The major pollutants in the influent were identified as hydrocarbons, aldehydes, heterocyclic matters, amines, alcohols, phenols, ketones, etc. by GC-MS analysis, while only heterocyclic compounds, ketones, and esters were detected in the effluent after HA-A/O treatment. Bacteria belonging to phyla Chloroflexi, Proteobacteria, and Bacteroidetes were highly enriched in the system. The predominant genera in HA, anoxic, and oxic tanks were Anaerolineaceae uncultured and Desulfobacter, Blastocatella and Anaerolineaceae uncultured, Saprospiraceae uncultured and Nitrosomonadaceae uncultured, respectively. The sulfate-reducing bacteria Desulfobacter, Desulfofustis and Desulfomicrobium were detected only in HA reactor. The ammonium-oxidizing bacteria Nitrosomonadaceae and Nitrosomonas and nitrite-oxidizing bacteria Nitrospira were highly enriched in A/O reactor, which is consistent with the good nitrification performance.