AFM detection of biophysical characteristics of specific regulatory T Cells after allogeneic hematopoietic stem cell transplantation

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• 作者：Xu Wang (1)
  Zhenyi Jin (2)
  Xinyu Wang (2)
  Shaohua Chen (2)
  Lijian Yang (2)
  Kanger Zhu (3)
  Xiuli Wu (1) (2)
  Yangqiu Li (1) (2)
  
  1. Key Laboratory for Regenerative Medicine of Ministry of Education ; Jinan University ; Guangzhou ; 510632 ; China
  2. Institute of Hematology ; Jinan University ; Guangzhou ; 510632 ; China
  3. Department of Hematology ; First Affiliated Hospital ; Jinan University ; Guangzhou ; 510632 ; China
  
• 关键词：Atomic force microscopy ; Regulatory T cells ; Graft versus host disease ; Allogeneic hematopoietic stem cell transplantation
• 刊名：BMC Biophysics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：1,701 KB
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• 刊物主题：Biophysics and Biological Physics;
• 出版者：BioMed Central
• ISSN：2046-1682

文摘

Background Based on the dynamics of antigen recognition from T cell receptor complex and T cell activation, different function of T cells may present different morphology feature, at least at molecular level. Regulatory T (Treg) cells play a critic role in regulation the onset of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Little is known about their morphology feature which may relate to the regulating function in GVHD. Results In this study, we detected the biophysical architectural changes of specific Treg cells subsets after allo-HSCT using biological atomic force microscopy to characterize their biological characteristics and improve our understanding of cell structure-function relationships in GVHD. There were dramatic overall shape and surface membrane deformations of the Treg cells associated with patients at GVHD onset or without GVHD. The Treg cells at GVHD onset or without GVHD could be distinguished by the morphologic parameters of morphology, membrane nanostructure, and membrane pore. These AFM parameters of Treg cells morphology differed obviously between GVHD and without GVHD. The multiple irregular microspikes could be observed on the surface of Treg cells without GVHD. Conclusions The remarkable biological morphology changes in the cell membrane structure of Treg cells after allo-HSCT might be related to the function of Treg cells which could inhibit the occurrence and development of GVHD.