A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault

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• 作者：Shanan S. Tobe ; Yuvaneswari C. Swaran…
• 关键词：Direct PCR ; Sexual assault ; Semen ; Seminal fluid ; Differential isolation ; Spermatozoa
• 刊名：International Journal of Legal Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：131
• 期：1
• 页码：87-94
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Forensic Medicine; Medical Law; Medicine/Public Health, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1437-1596
• 卷排序：131

文摘

Sexual assault samples are some of the most common samples encountered in forensic analysis. These samples can require a significant time investment due to differential extraction processes. We report on the first record of successful direct amplification of semen for STR analysis. Neat seminal fluid, dilutions ranging from 1:5 to 1:160 and GEDNAP samples were successfully amplified using a direct method. A mild differential isolation technique to enrich spermatozoa was developed and successfully implemented to separate and directly amplify a mixture of semen and female epithelial cells. Aliquots of samples subjected to the differential isolation protocol were stained with Haemotoxylin and Eosin for sperm scoring. Samples stained after PCR showed a complete lack of intact spermatozoa demonstrating that the cells are lysed during the PCR process. This paper demonstrates the potential to incorporate direct PCR in cases of sexual assault to more rapidly obtain results and achieve a higher sensitivity.