Variation and expression of KAP9.2 gene affecting cashmere trait in goats

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• 作者：X. Wang (1)
  Z. D. Zhao (1)
  H. R. Xu (1)
  L. Qu (2)
  H. B. Zhao (3)
  T. Li (1)
  Z. Y. Zhang (1)
  
• 关键词：Cashmere goat ; SNP ; KAP9.2 gene ; Expression
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：39
• 期：12
• 页码：10525-10529
• 全文大小：295KB
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• 作者单位：X. Wang (1)
  Z. D. Zhao (1)
  H. R. Xu (1)
  L. Qu (2)
  H. B. Zhao (3)
  T. Li (1)
  Z. Y. Zhang (1)
  
  1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science & Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  2. Life Science Research Center, Yuling University, Yulin, 719000, Shaanxi, People’s Republic of China
  3. Institute of Animal Science and Veterinary, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, People’s Republic of China
  
• ISSN：1573-4978

文摘

Keratin-associated proteins 9.2 (KAP9.2) gene encodes one of the ultra high sulfur KAPs. Variation in KAP genes may affect the structure of KAPs and hence cashmere characteristics. In order to test the association between the polymorphism of KAP9.2 gene and cashmere trait, DNA sequencing was used to detect a novel C/T polymorphism of KAP9.2 gene from a genomic DNA pool. The mutation could be recognized by Pst I restriction enzyme. To Shanbei white cashmere goat, Inner Mongolia white cashmere goat and Guanzhong dairy goat, the genotypic frequencies of TT, TC and CC from total 1,236 animals were as follows: 0.047, 0.519 and 0.434; 0.180, 0.592 and 0.228; 0.431, 0.544 and 0.025. The allelic frequencies of T and C were 0.307 and 0.693; 0.476 and 0.524; 0.703 and 0.297, respectively, in breeds mentioned above. The frequency of C allele between cashmere and dairy goat was significant (P?<?0.01). To provide support for the hypothesis that SNP 586 was responsible for KAP9.2 expression, quantitative real-time PCR analysis revealed that the expression level of KAP9.2 was reduced in individuals bearing genotype CC compared with TT individuals, suggesting that C was the nucleotide causing decreased expression of KAP9.2 or was in linkage disequilibrium with the causative SNP. The 586C/T SNP found in this study might control translation or stability of KAP9.2 mRNA, which would be beneficial for marker assistant selection in cashmere goat breeding.