A new method for improving the accuracy of miRNA detection with NaYF₄:Yb,Er upconversion nanoparticles

详细信息    查看全文

• 作者：Lanlan Mao ; Zhuoxuan Lu ; Nongyue He ; Liming Zhang ; Yan Deng…
• 关键词：microRNAs detection ; NaYF4 ; Yb ; Er ; reference standard ; base stacking hybridization
• 刊名：Science China Chemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：60
• 期：1
• 页码：157-162
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry/Food Science, general;
• 出版者：Science China Press
• ISSN：1869-1870
• 卷排序：60

文摘

MicroRNAs (miRNAs) are small noncoding RNAs, which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis. So far, many miRNAs detection methods require polymerase chain reaction (PCR) amplification following reverse transcription of miRNAs. These processes are complicated and time-consuming. In this work, we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF₄:Yb,Er upconversion nanoparticles. In this method, the fluorescence of NaYF₄:Yb,Er upconversion nanoparticles were functionalized as a reference standard, which can improve the accuracy of miRNA detection. On the basis of these findings, we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.