Detection and characterization of diverse alpha- and betacoronaviruses from bats in China

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• 作者：Lin Xu ; Fuqiang Zhang ; Weihong Yang ; Tinglei Jiang ; Guanjun Lu…
• 关键词：bats ; Alphacoronavirus ; Betacoronavirus ; diversity
• 刊名：Virologica Sinica
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：31
• 期：1
• 页码：69-77
• 全文大小：519 KB
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• 作者单位：Lin Xu (1)
  Fuqiang Zhang (2)
  Weihong Yang (3)
  Tinglei Jiang (4)
  Guanjun Lu (4)
  Biao He (1)
  Xingyu Li (1)
  Tingsong Hu (2)
  Gang Chen (2)
  Yun Feng (3)
  Yuzhen Zhang (3)
  Quanshui Fan (2)
  Jiang Feng (4)
  Hailin Zhang (3)
  Changchun Tu (1) (5)
  
  1. Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China
  2. Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China
  3. Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China
  4. School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China
  5. Jiangsu Co-innovation Center for Prevention and Control of Important, Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China
  
• 刊物主题：Virology; Medical Microbiology; Oncology; Biochemistry, general; Microbiology; Microbial Genetics and Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1995-820X

文摘

Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. Keywords bats Alphacoronavirus Betacoronavirus diversity ORCID: 0000-0002-8134-7502