Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using next-generation sequencing technology

详细信息    查看全文

• 作者：Ke Wang (1)
  Shuhuan Zhang (2)
  Dengqiang Wang (2)
  Miaomiao Xin (2)
  Jinming Wu (2)
  Qingliang Sun (2)
  Hao Du (2)
  Chengyou Wang (2)
  Jun Huang (2)
  Qiwei Wei (1) (2)
  
  1. Wuxi Fisheries College ; Nanjing Agricultural University ; Wuxi ; 214081 ; China
  2. Yangtze River Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; Wuhan ; 430223 ; China
  
• 关键词：Hucho bleekeri ; Microsatellite ; Endangered species
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：263-267
• 全文大小：309 KB
• 参考文献：1. Cheng W, Wang D, Du H, Zhang S, Wei Q (2013) Isolation and characterization of 23 microsatellite loci in the Chinese sucker ( / Myxocyprinus asiaticus). Conservation Genet Resour 5:375鈥?77 CrossRef
  2. Hu M, Wang Y, Cao L, Xiong B (2008) Threatened fishes of the world: / Hucho bleekeri Kimura, 1934 (Salmonidae). Environ Biol Fish 82:385鈥?86 CrossRef
  3. Salah MA, Iciar M (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25(22):4692鈥?693 CrossRef
  4. Wang S (1998) China red data book of endangered animals-Pisces. Science Press, Beijing
  5. Zhu B, Liao X, Shao Z, Rosenthal H, Chang J (2005) Isolation and characterization of microsatellites in Chinese sturgeon, / Acipenser sinensis. Mol Ecol Notes 5:888鈥?92 CrossRef
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

In this study, we developed 27 polymorphic microsatellite loci in Hucho bleekeri, a glacial relict and freshwater resident salmonid fish in China. The number of alleles varied from 2 to 8 for each primer set. The observed and expected heterozygosity ranged from 0.250 to 0.906 and 0.508 to 0.845, respectively. The polymorphic information content ranged from 0.371 to 0.808. These microsatellite loci should be useful to study population genetics, paternity identification, speciation and adaptive evolution of this lineage.