Fabrication and characterization of chitosan microcarrier for hepatocyte culture

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• 作者：Chen Wu ; Jilun Pan ; Zhiming Bao and Yaoting Yu
• 刊名：Journal of Materials Science Materials in Medicine
• 出版年：2007
• 出版时间：November, 2007
• 年：2007
• 卷：18
• 期：11
• 页码：2211-2214
• 全文大小：284.1 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biomaterials
  Characterization and Evaluation Materials
  Polymer Sciences
  Metallic Materials
  Ceramics,Glass,Composites,Natural Materials
  Surfaces and Interfaces and Thin Films
  
• 出版者：Springer Netherlands
• ISSN：1573-4838

文摘

Using chitosan as raw materials, a suitable size (300–500 μm) of porous microcarrier was fabricated by suspension crosslinking and lyophilizing method, which made the carrier has an average pore size of 50 μm and 86% porosity. The microcarrier was modified with lactose and maltose respectively. Various factors that influenced the preparation of microcarrier were studied and the reaction conditions were optimized. Rat hepatocytes cultured on modified microcarrier retained a spherical shape which is similar to those in vivo and formed aggregates. The metabolic activities of cells on lactose-modified were higher than those on maltose-modified microcarrier. The highest albumin secretion reached 54.8 μg/106 cells/d, and the highest urea synthesis reached 4.65 μmol/106cells/d, which may be promoted by the formation of cellular aggregates. In conclusion, lactose-modified porous microcarrier is promising scaffold for hepatocytes culture.