Determination of microcystin-LR with a glassy carbon impedimetric immunoelectrode modified with an ionic liquid and multiwalled carbon nanotubes
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- 作者：Xiulan Sun (1)
Lu Guan (1)
Hui Shi (1)
Jian Ji (1)
Yinzhi Zhang (1)
Zaijun Li (2) - 关键词：Microcystin ; LR ; Impedance immunosensor ; Ionic liquid ; Multiwalled carbon nanotubes
- 刊名：Microchimica Acta
- 出版年：2013
- 出版时间：2 - January 2013
- 年：2013
- 卷：180
- 期：1
- 页码：75-83
- 全文大小：402KB
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Lu Guan (1)
Hui Shi (1)
Jian Ji (1)
Yinzhi Zhang (1)
Zaijun Li (2)
1. The Key Laboratory of Food Science and technology, School of Food Science, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu province, 214122, China
2. School of Chemical and Materials Engineering, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu province, 214122, China - ISSN：1436-5073
文摘
We report on a sensitive, simple, label-free impedance-based immunoelectrode for the determination of microcystin-LR (MCLR). The surface of the electrode was modified with a composite made from multiwalled carbon nanotubes and an ionic liquid, and with immobilized polyclonal antibody against MCLR. Cyclic voltammetry and impedance spectroscopy were applied to characterize the modified electrode. It is found that the multi-walled carbon nanotubes act as excellent mediators for the electron transfer between the electrode and dissolved hexacyanoferrate redox pair, while the ionic liquid renders it biocompatible. The method exhibits a wide linear range (0.005?μg?L-1 to 1.0?μg?L-1), a low detection limit (1.7?ng?L-1) and a long-term stability of around 60?days. The ionic liquid 1-amyl-2,3-dimethylimidazolium hexafluorophosphate gave the best impedimetric response. The new immunoelectrode is sensitive, stable, and easily prepared. It has been successfully applied to the determination of MCLR in water samples. Figure The immunosensor, modified with a nanocomposite of room temperature ionic liquid- multiwalled carbon nanotube, was applied to detect MCLR. The method exhibits a wide linear range (0.005?μg·L? to 1.0?μg·L?), a low detection limit (1.7 ng·L-1) and a long-term stability of around 60 days.