Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

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• 作者：Juan Bai (1)
  Xinhui Chen (1)
  Kangfu Jiang (1)
  Basit Zeshan (1)
  Ping Jiang (1) (2)
  
  1. Key Laboratory of Animal Diseases Diagnostic and Immunology ; Ministry of Agriculture ; College of Veterinary Medicine ; Nanjing Agricultural University ; Nanjing ; 210095 ; China
  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses ; Yangzhou ; China
  
• 关键词：Encephalomyocarditis virus (EMCV) ; VP1 ; McAbs ; Epitopes
• 刊名：Virology Journal
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：1,609 KB
• 参考文献：1. Carocci, M, Bakkali-Kassimi, L (2012) The encephalomyocarditis virus. Virulence 3: pp. 351-367 CrossRef
  2. Gelmetti, D, Meroni, A, Brocchi, E, Koenen, F, Cammarata, G (2006) Pathogenesis of encephalomyocarditis experimental infection in young piglets: a potential animal model to study viral myocarditis. Vet Res 37: pp. 15-23 CrossRef
  3. Grobler, DG, Raath, JP, Braack, LE, Keet, DF, Gerdes, GH, Barnard, BJ, Kriek, NP, Jardine, J, Swanepoel, R (1995) An outbreak of encephalomyocarditis-virus infection in free-ranging African elephants in the Kruger National Park. Onderstepoort J Vet Res 62: pp. 97-108
  4. Hubbard, GB, Soike, KF, Butler, TM, Carey, KD, Davis, H, Butcher, WI, Gauntt, CJ (1992) An encephalomyocarditis virus epizootic in a baboon colony. Lab Anim Sci 42: pp. 233-239
  5. Jones, P, Mahamba, C, Rest, J, Andre, C (2005) Fatal inflammatory heart disease in a bonobo (Pan paniscus). J Med Primatol 34: pp. 45-49 CrossRef
  6. Juncker-Voss, M, Prosl, H, Lussy, H, Enzenberg, U, Auer, H, Lassnig, H, Muller, M, Nowotny, N (2004) Screening for antibodies against zoonotic agents among employees of the Zoological Garden of Vienna, Schonbrunn, Austria. Berl Munch Tierarztl Wochenschr 117: pp. 404-409
  7. LaRue, R, Myers, S, Brewer, L, Shaw, DP, Brown, C, Seal, BS, Njenga, MK (2003) A wild-type porcine encephalomyocarditis virus containing a short poly(C) tract is pathogenic to mice, pigs, and cynomolgus macaques. J Virol 77: pp. 9136-9146 CrossRef
  8. Reddacliff, LA, Kirkland, PD, Hartley, WJ, Reece, RL (1997) Encephalomyocarditis virus infections in an Australian zoo. J Zoo Wildl Med 28: pp. 153-157
  9. Billinis, C, Paschaleri-Papadopoulou, E, Psychas, V, Vlemmas, J, Leontides, S, Koumbati, M, Kyriakis, SC, Papadopoulos, O (1999) Persistence of encephalomyocarditis virus (EMCV) infection in piglets. Vet Microbiol 70: pp. 171-177 CrossRef
  10. Denis, P, Liebig, HD, Nowotny, N, Billinis, C, Papadopoulos, O, O鈥橦ara, RS, Knowles, NJ, Koenen, F (2006) Genetic variability of encephalomyocarditis virus (EMCV) isolates. Vet Microbiol 113: pp. 1-12 CrossRef
  11. Dea, S, Bilodeau, R, Sauvageau, R, Martineau, GP (1991) Outbreaks in Quebec pig farms of respiratory and reproductive problems associated with encephalomyocarditis virus. J Vet Diagn Invest 3: pp. 275-282 CrossRef
  12. Koenen, F, Declercq, K, Lefebvre, J, Strobbe, R (1994) Reproductive failure in sows following experimental-infection with a Belgian Emcv isolate. Vet Microbiol 39: pp. 111-116 CrossRef
  13. Zhang, GZ, Zhao, JX, Wang, M (2007) Serological survey on prevalence of antibodies to avian paramyxovirus serotype 2 in China. Avian Dis 51: pp. 137-139 CrossRef
  14. Sandwyk, JH, Bennett, NC, Swanepoel, R, Bastos, AD (2013) Retrospective genetic characterisation of Encephalomyocarditis viruses from African elephant and swine recovers two distinct lineages in South Africa. Vet Microbiol 162: pp. 23-31 CrossRef
  15. Palmenberg, AC, Kirby, EM, Janda, MR, Drake, NL, Duke, GM, Potratz, KF, Collett, MS (1984) The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Res 70: pp. 2969-2985 CrossRef
  16. Whitton, JL, Cornell, CT, Feuer, R (2005) Host and virus determinants of picornavirus pathogenesis and tropism. Nat Rev Microbiol 3: pp. 765-776 CrossRef
  17. Kobasa, D, Mulvey, M, Lee, JS, Scraba, DG (1995) Characterization of Mengo-Virus Neutralization Epitopes.2. Infection of Mice with an Attenuated Virus. Virology 214: pp. 118-127 CrossRef
  18. Sin, JI, Sung, JH, Suh, YS, Lee, AH, Chung, JH, Sung, YC (1997) Protective immunity against heterologous challenge with encephalomyocarditis virus by VP1 DNA vaccination: effect of coinjection with a granulocyte-macrophage colony stimulating factor gene. Vaccine 15: pp. 1827-1833 CrossRef
  19. Lin, X, Zhao, J, Qian, J, Mao, Y, Pan, J, Li, L, Peng, H, Luo, Y, Yan, J (2010) Identification of immunodominant B- and T-cell combined epitopes in outer membrane lipoproteins LipL32 and LipL21 of Leptospira interrogans. Clin Vaccine Immunol 17: pp. 778-783 CrossRef
  20. Peng, WP, Hou, Q, Xia, ZH, Chen, D, Li, N, Sun, Y, Qiu, HJ (2008) Identification of a conserved linear B-cell epitope at the N-terminus of the E2 glycoprotein of Classical swine fever virus by phage-displayed random peptide library. Virus Res 135: pp. 267-272 CrossRef
  21. Song, Y, Zhou, Y, Li, Y, Wang, X, Bai, J, Cao, J, Jiang, P (2012) Identification of B-cell epitopes in the NSP1 protein of porcine reproductive and respiratory syndrome virus. Vet Microbiol 155: pp. 220-229 CrossRef
  22. Pettersen, EF, Goddard, TD, Huang, CC, Couch, GS, Greenblatt, DM, Meng, EC, Ferrin, TE (2004) UCSF Chimera鈥揳 visualization system for exploratory research and analysis. J Comput Chem 25: pp. 1605-1612 CrossRef
  23. Viudes, A, Perea, S, Lopez-Ribot, JL (2001) Identification of continuous B-cell epitopes on the protein moiety of the 58-kiloDalton cell wall mannoprotein of Candida albicans belonging to a family of immunodominant fungal antigens. Infect Immun 69: pp. 2909-2919 CrossRef
  24. Yin, CH, Qin, LT, Sun, MY, Gao, YL, Qi, XL, Gao, HL, Wang, YQ, Jang, LL, Wang, XM (2013) Identification of a linear B-Cell epitope on avian reovirus protein sigmaC. Virus Res 178: pp. 530-534 CrossRef
  25. Yu, H, Jiang, LF, Fang, DY, Yan, HJ, Zhou, JJ, Zhou, JM, Liang, Y, Gao, Y, Zhao, W, Long, BG (2007) Selection of SARS-coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice. Virology 359: pp. 264-274 CrossRef
  26. Bai, J, Jiang, K, Zeshan, B, Wang, X, Li, Y, Jiang, P (2012) Inhibition of encephalomyocarditis virus replication by shRNA targeting 1D and 3AB genes in vitro and in vivo. Virus Genes 44: pp. 183-190 CrossRef
  27. Bai, J, Jiang, K, Zhang, L, Wang, X, Li, Y, Jiang, P (2012) Protective efficacy of adenovirus-mediated small interfering RNAs against encephalomyocarditis virus challenge in mice. J Virol Methods 185: pp. 204-212 CrossRef
  28. Suh, YS, Ha, SJ, Lee, CH, Sin, JI, Sung, YC (2001) Enhancement of VP1-specific immune responses and protection against EMCV-K challenge by co-delivery of IL-12 DNA with VP1 DNA vaccine. Vaccine 19: pp. 1891-1898 CrossRef
  29. Denis, P, Koenen, F (2003) Molecular analysis of the capsid coding region of a virulent encephalomyocarditis virus isolate after serial cell passages and assessment of its virulence. Arch Virol 148: pp. 903-912 CrossRef
  30. Jun, HS, Kang, Y, Notkins, AL, Yoon, JW (1997) Gain or loss of diabetogenicity resulting from a single point mutation in recombinant encephalomyocarditis virus. J Virol 71: pp. 9782-9785
  31. Nelsen-Salz, B, Zimmermann, A, Wickert, S, Arnold, G, Botta, A, Eggers, HJ, Kruppenbacher, JP (1996) Analysis of sequence and pathogenic properties of two variants of encephalomyocarditis virus differing in a single amino acid in VP1. Virus Res 41: pp. 109-122 CrossRef
  32. Zhu, S, Ge, XN, Gong, XW, Guo, X, Chen, YH, Yang, HC (2011) Alteration of encephalomyocarditis virus pathogenicity due to a mutation at position 100 of VP1. Sci China Life Sci 54: pp. 535-543 CrossRef
  33. Jnaoui, K, Minet, M, Michiels, T (2002) Mutations that affect the tropism of DA and GDVII strains of Theiler鈥檚 virus in vitro influence sialic acid binding and pathogenicity. J Virol 76: pp. 8138-8147 CrossRef
  34. Guy, M, Chilmonczyk, S, Cruciere, C, Eloit, M, Bakkali-Kassimi, L (2009) Efficient infection of buffalo rat liver-resistant cells by encephalomyocarditis virus requires binding to cell surface sialic acids. J Gen Virol 90: pp. 187-196 CrossRef
  
• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X

文摘

Background Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity. In this study, 10 monoclonal antibodies (McAbs) against EMCV VP1 were screened and identified. Results Epitope mapping results indicated that McAbs (6E11, 7A7, 7C9) specifically recognized the linear epitopes V(2)ENAEK(7), McAbs (1D1, 2A2, 5A1, 5A11, 5G1) recognized the epitope F(19)VAQPVY(25), and McAbs 1G8 and 3A9 recognized P(42)IGAFTVK(49). Protein sequence alignment of VP1 with 16 EMCV isolates indicated that the epitope F(19)VAQPVY(25) was conserved in all the reference strains. The epitopes P(42)IGAFTVK(49) and V(2)ENAEK(7) only had 1 or 2 variable amino acid among the reference strains. The 3D model analysis results showed that these epitopes presented as spheres were shown within the context of the complete particle. Conclusions In this study, ten McAbs against EMCV VP1 were developed and three B-cells epitopes (2-7aa, 19-25aa and 42-49aa) were defined in VP1. All the results herein will promote the future investigations into the function of VP1 of EMCV and development of diagnostic methods of EMCV.