Antiviral activities of natural compounds derived from traditional chinese medicines against porcine circovirus type 2 (PCV2)

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• 作者：Na Sun (1)
  Tong Yu (1)
  Jun-Xing Zhao (1)
  Yao-Gui Sun (1)
  Jun-Bing Jiang (1)
  Zhi-Bian Duan (1)
  Wen-Kui Wang (1)
  Yuan-Liang Hu (2)
  Hai-Min Lei (3)
  Hong-Quan Li (1)
  
  1. College of Animal Science and Veterinary Medicine ; Shanxi Agricultural University ; Taigu ; China
  2. Institute of Traditional Chinese Veterinary Medicine ; College of Veterinary Medicine ; Nanjing Agricultural University ; Nanjing ; China
  3. School of Chinese Pharmacy ; Beijing University of Chinese Medicine ; Beijing ; China
  
• 关键词：PCV2 ; matrine ; scutellarin ; antiviral ; in vitro
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：20
• 期：1
• 页码：180-187
• 全文大小：433 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Twenty natural compounds isolated from traditional Chinese medicines were examined for their antiviral activities against PCV2 in vitro. Antiviral activity was analyzed by the assays of blocking of virus entry, inhibition of virus replication and inactivation of virus particles in the virus suspension. 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) for each compound were determined by using the MTT method and immunofluorescence assay (IFA), respectively. The results obtained from the inhibition of virus replication and inactivation of virus particles showed that the maximum inhibition rates for matrine and scutellarin were 57 and 72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virus entry and inhibition of virus replication demonstrated that both aesculetin and arecoline hydrochloride stimulated PCV2 replication, with the maximum stimulation rate of 387.3 and 219.4%. In summary, both matrine and scutellarin had antiviral activity against PCV2, while aesculetin and arecoline hydrochloride promoted PCV2 infection replication in vitro.