Escherichia coli (XL1-BLUE) for continuous fermentation of bioHb>2b> and its separation by polyimide membrane

详细信息	查看全文 | 推荐本文 |

• 作者：P. Bakonyi^a ; ^{bakonyip@almos.uni-pannon.hu} ; ^{bakonyipeter85@gmail.com} ; N. Nemestó ; thy^a ; J. Ramirez^b ; G. Ruiz-Filippi^b ; K. B&#233 ; lafi-Bakó ; ^a
• 关键词：Escherichia coli ; Biohydrogen ; Continuous fermentation ; Membrane separation
• 刊名：International Journal of Hydrogen Energy
• 出版年：2012
• 期刊代码：42_03603199
• 类别：ch
• 出版时间：April, 2012
• 卷：37
• 期：7
• 页码：5623-5630
• 文件大小：524 K

摘要

In this work continuous hydrogen production by Escherichia coli (XL1-BLUE) and its purification by membrane gas separation were studied. Firstly, a kinetic investigation was performed on formate supplemented broth in order to determine exponential growth phase (5-7聽h) while the most intense hydrogen fermentation takes place. Furthermore, important process design parameters such as saturation constant and maximal growth rate were calculated (Kb>Sb>聽=聽0.77聽g聽l^鈭?, 渭b>maxb>聽=聽0.39聽h^鈭?). Afterward, based on the kinetic study, continuous hydrogen fermentations using cultures of E. coli (XL1-BLUE) were carried out in a CSTR reactor configuration applying various hydraulic retention times (HRT) related to both exponential and stationary growth period (5聽h, 7聽h, 9聽h). The results indicated that highest hydrogen yield (0.26聽mmol Hb>2b>/mmol formate added) and productivity (5.1聽mmol Hb>2b>聽l^鈭?聽d^鈭?) could be achieved by applying HRT聽=聽7聽h that does not allow the living cells to reach stationary phase. In addition to hydrogen production, the concentration of bioHb>2b> by polyimide membrane under different operational circumstances was investigated using pure and mixed gases, as well. The results of single gas experiments indicated that increasing the temperature has positive effect on separation efficiency. Moreover, the influence of retentate and feed flow ratio (Qb>Rb>/Qb>Fb>) was studied applying binary Hb>2b>/COb>2b> gaseous mixture and it was found that polyimide membrane has high potential for Hb>2b> purification since 18%increase in Hb>2b> concentration and 22%decrease in COb>2b> content could be attained in the permeate by a one-step separation process.