Prognostic significance of MCM7 expression in the bronchial brushings of patients with non-small cell lung cancer (NSCLC)

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• 作者：Yi-Zhen Liu^a ; Yan-Yi Jiang^a ; Jia-Jie Hao^a ; Shan-Shan Lu^b ; Tong-Tong Zhang^a ; Li Shang^a ; Jian Cao^b ; Xin Song^c ; Bo-Shi Wang^a ; Yan Cai^a ; Qi-Min Zhan^a ; Ming-Rong Wang^a ; ^{wangmr07@gmail.com}
• 关键词：Prognosis ; Non-small cell lung cancer ; Bronchial brushings ; Biomarker ; MCM7 ; Ki67 ; EGFR
• 刊名：Lung Cancer
• 出版年：2012
• 期刊代码：187_01695002
• 类别：med
• 出版时间：July, 2012
• 卷：77
• 期：1
• 页码：176-182
• 文件大小：1005 K

摘要

lass="h4">Purpose

To identify potential biomarkers for the prognosis of non-small cell lung cancer (NSCLC) patients by using bronchial brushing specimens.

lass="h4">Methods

The expression of MCM7, Ki67 and EGFR was evaluated in 494 NSCLC tissues and 174 bronchial brushings using immunohistochemical and immunocytochemical techniques. Associations between protein expression and clinico-pathologic parameters were assessed, and the impact on overall survival (OS) was analyzed.

lass="h4">Results

High expression of MCM7, Ki67 and EGFR was detected in 33.3%, 23.5%and 12.7%of tissues and in 52.4%, 52.7%and 20.6%of bronchial brushings, respectively. Expression of MCM7 and Ki67 was associated with squamous cell carcinoma (SCC) in both tissues and bronchial brushings (MCM7: m>Pm> = 0.0007, 0.00003; Ki67: m>Pm> < 0.00001, 0.00001). Overexpression of MCM7 in tumor tissues was detected more frequently in poorly differentiated tumors (m>Pm> = 0.0120) and non-bronchioloalveolar carcinomas (non-BACs) (m>Pm> = 0.0238). EGFR overexpression was observed in tissues of larger tumors (m>Pm> = 0.00004) and in bronchial brushings at later stage (m>Pm> = 0.0262). Kaplan-Meier curves indicated that patients with overexpression of MCM7 or Ki67 had a poorer OS compared to those with low expression for all stages (m>Pm> < 0.00001, 0.0233) and early-stages (m>Pm> < 0.00001, 0.0032). In particular, the patients with MCM7 overexpression in bronchial brushings had a poorer prognosis (m>Pm> = 0.0045). Multivariate Cox regression analysis showed that MCM7 was an independent prognostic indicator both in tissue samples and bronchial brushings.

lass="h4">Conclusions

Our data suggest that MCM7 and Ki67 in tumor tissues may be potential markers of a poor prognosis for NSCLC patients. MCM7 in bronchial brushings also showed an independent prognostic value, which may be useful when biopsies are unavailable.