Composition and cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica)

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• 作者：Zhenfei Peng^a ; ^b ; Min Liu^a ; ^b ; Zhexiang Fang^a ; Jiulin Wu^a ; Qiqing Zhang^a ; ^c ; ^{zhangqiq@126.com}
• 关键词：Laminaria japonica polysaccharide ; Structure characterization ; Antitumor activity ; Low cytotoxicity
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 August, 2012
• 卷：89
• 期：4
• 页码：1022-1026
• 文件大小：481 K

摘要

A novel polysaccharide WPS-2-1, with an average molecular weight of 80 kDa, was purified from aqueous extracts of Laminaria japonica. Monosaccharides analysis revealed that WPS-2-1 was composed of mannose, rhamnose and fucose with a molar ratio of 1.0:2.3:1.2. Analysis by periodate oxidation-Smith degradation indicated that WPS-2-1 had a backbone of array by (1 鈫?#xA0;4)-glycosidic linkages. Cytotoxicity assay showed that WPS-2-1 presented significantly higher antitumor activities against A375 and BGC823 cells with a dose-dependent manner, and exhibited lower cytotoxicity to vascular smooth muscle cells. The results suggested that WPS-2-1 should be explored as a potential antitumor agent with low toxicity.