DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide

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• 作者：Rebeca Santiso^a ; ^b ; Marí ; a Tamayo^a ; ^b ; Jaime Gos&aacute ; lvez^c ; Steve Johnston^d ; Alfonso Mariñ ; o^e ; Carlos Fern&aacute ; ndez^f ; Carlos Losada^f ; José ; Luis Fern&aacute ; ndez^a ; ^b ; ^{Jose.Luis.Fernandez.Garcia@sergas.es} ; ^{joseluis.fernandez@cog.es}
• 关键词：Sperm ; DNA fragmentation ; Dynamic assay ; SCD test ; Cryptic damage
• 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
• 出版年：2012
• 期刊代码：115_00275107
• 类别：bio
• 出版时间：1 June, 2012
• 卷：734
• 期：1-2
• 页码：41-49
• 文件大小：1257 K

摘要

Sperm DNA fragmentation (SDF) is not a static seminal parameter, since the longevity of sperm DNA decreases progressively with time following ejaculation or thawing. While the dynamics of SDF is a species-specific characteristic, in the case of humans, there is still significant variation within patients. To evaluate the suitability of the dynamic SDF assay to assess the adverse effects of agents that cause genetic damage, fresh semen samples from different donors were exposed in vitro to (1) increasing acute doses of ionizing radiation, (2) elevated temperature (41 掳C and 45 掳C), (3) acidic pH (pH 4) and (4) the nitric oxide (NO) donor sodium nitroprusside (SNP). Sperm DNA fragmentation was analyzed after an incubation period of chronic (24 h), or acute (1 h) exposure to each treatment followed by incubation at 37 掳C over a period of 24 h. SDF was assessed using the sperm chromatin dispersion (SCD) test. Dynamic SDF for each treatment was analyzed using Kaplan-Meier survival curves. All agents, except for ionizing radiation, accelerated SDF kinetics following chronic exposure over a 24 h period. Transient exposure to NO and heat but not acidic pH increased the basal (T0) level of SDF. Despite the removal of the three toxicants, the remaining sperm following acute exposure showed a decrease in their expected DNA longevity. It is concluded that the assessment of sperm DNA fragmentation dynamics is an effective methodological approach for revealing latent damage associated with toxicants that is not initially expressed following a single initial observation of SDF.