Deep 16S rRNA metagenomics and quantitative PCR analyses of the premature infant fecal microbiota

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• 作者：Silvia Arboleya^a ; Li Ang^b ; Abelardo Margolles^a ; Li Yiyuan^b ; Zhang Dongya^b ; Xiao Liang^b ; Gonzalo Solí ; s^c ; Nuria Fern&aacute ; ndez^d ; Clara G. de los Reyes-Gavil&aacute ; n^a ; Miguel Gueimonde^a ; ^{mgueimonde@ipla.csic.es}
• 关键词：Intestinal microbiota ; Preterm infants ; Neonates ; Metagenomics
• 刊名：Anaerobe
• 出版年：2012
• 期刊代码：158_10759964
• 类别：bio
• 出版时间：June, 2012
• 卷：18
• 期：3
• 页码：378-380
• 文件大小：275 K

摘要

Metagenomic studies on the gut microbiota of preterm infants are scarce. We characterized the microbiota of 10 days-old neonates by deep 16S rRNA gene metagenomic analysis and compare the results with those obtained by qPCR. Both techniques lead to similar conclusions, allowing differentiating between preterm and full-term infants.