Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment

详细信息	查看全文 | 推荐本文 |

• 作者：Viera Habalov&aacute ; ^a ; Lucia Klim膷&aacute ; kov&aacute ; ^a ; Jozef 沤idzik^a ; ^{jozef.zidzik@upjs.sk} ; Peter Vasov膷&aacute ; k^c ; Pavol Kristian^b ; Ivana Valkov&aacute ; ^b ; Ivan Schré ; ter^b ; J&aacute ; n &Scaron ; alagovi膷^a
• 关键词：Hepatitis C ; IL28B polymorphism ; Small amplicon ; Melting analysis
• 刊名：Molecular and Cellular Probes
• 出版年：2012
• 期刊代码：104_08908508
• 类别：imm
• 出版时间：August, 2012
• 卷：26
• 期：4
• 页码：159-163
• 文件大小：275 K

摘要

Background & aim

Hepatitis C is a liver disease caused by the hepatitis C virus. Interferon and ribavirin combination therapy has been a standard treatment of chronic hepatitis C. But only about 50%of patients have positive response to treatment and achieve so called sustained virological response. Recent studies indicate association of several single nucleotide polymorphisms near IL28B gene and response of hepatitis C patients to combined interferon/ribavirin treatment. In this study, rapid, specific and cost-effective small amplicon genotyping method for the two clinically important polymorphisms, rs12979860 C聽>聽T and rs8099917 T聽>聽G, near the IL28B gene is described.

Methods

The distribution of genotypes of 181 HCV-uninfected Slovak Caucasians was analyzed using this novel method, based on a real-time melting analysis of the small amplicon.

Results and conclusions

The frequency of wild-type (TT) homozygotes for rs8099917 was 66.30%, frequency of heterozygotes (TG) was 30.94%and we found only 2.76%subjects homozygous for risk G allele (allelic frequencies: T聽=聽81.77%, G聽=聽18.23%) were found. The frequency of wild-type genotype (CC) for rs12979860 was 49.72%, frequencies of heterozygous (CT) and risk-allele homozygous genotypes (TT) were 39.78%and 10.50%, respectively (allele frequencies: C聽=聽69.61%, T聽=聽30.39%). Statistically significant differences in the distribution of the alleles between the men and the women were not found. The novel method developed in our laboratory proved to be simple and highly customizable.