- 作者：Efstathios Kotidis ; M.Sc. ; Ph.D.&lowast ; ; skotidis@med.auth.gr ; skotidis@gmail.com ; Theodosis Papavramidis ; Ph.D.&lowast ; ; Konstantinos Ioannidis ; M.D.&lowast ; ; George Koliakos ; Ph.D.&dagger ; ; Thomai Lazou&lowast ; ; Aggeliki Cheva ; Ph.D.&Dagger ; ; Nikolaos Michalopoulos ; M.D.&lowast ; ; Spiros Papavramidis ; Ph.D.&lowast ;
- 关键词：intra-abdominal hypertension ; oxidative stress ; rectus abdominis muscle ; SOD ; superoxide dismutase ; glutathione ; glutathione peroxidase ; glutathione reductase ; lipid peroxidation ; protein carbonyls ; pro-oxidant&ndash ; antioxidant balance
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：July, 2012
- 卷：176
- 期：1
- 页码：102-107
- 文件大小：158 K
Background
The aim of this study was to test the hypothesis that intra-abdominal hypertension alone could trigger such changes to the rectus abdominis muscle that would lead to an imbalance between oxidant production and antioxidant protection.Materials and Methods
Forty-five New Zealand white rabbits were divided into three groups and a rubber bag was implanted into their peritoneal cavity. In聽group A (n = 15), the bag was empty. In group B (n聽= 15), it was filled with normal saline to achieve an intra-abdominal pressure of over 12 mm Hg. In group C (n = 15), it was filled with lead equiponderant to the mean weight of the normal saline injected in group聽B. After 8 weeks, we measured in rectus abdominis muscle biopsies the lipid peroxidation products, the protein carbonyl content, the total glutathione and superoxide dismutase (SOD) concentration, the activity of glutathione reductase and glutathione peroxidase, and the pro-oxidant-antioxidant balance.
Results
The lipid peroxidation products were significantly higher in group B compared with both group A (P = 0.026) and group C (P < 0.001). The total protein carbonyl content was significantly higher in group B compared with both group A (P = 0.006) and group C (P < 0.001). No difference was found between the three groups in total glutathione (P = 0.735) and SOD (P = 0.410) concentration. Glutathione peroxidase activity was higher in groups B and C compared with group A (P = 0.05 and P = 0.003, respectively). Glutathione reductase activity was higher in group B compared with group A (P = 0.005) and group C (P = 0.001). The pro-oxidant antioxidant balance was higher in group B compared with the group A (P = 0.012).
Conclusions
Maintaining the IP over 12 mm Hg for 8 wk caused increased oxidative damage to both lipids and proteins with an increased pro-oxidant-antioxidant balance. In an attempt to compensate for this damage the muscle fibers increased their glutathione reductase and glutathione peroxidase activity.