Surface modified liposomes by mannosylated conjugates anchored via the adamantyl moiety in the lipid bilayer

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• 作者：Adela &Scaron ; timacp>ap> ; Suzana &Scaron ; egotap>bp> ; Maja Dutour Sikiri膰p>bp> ; Rosana Ribi膰p>cp> ; Leo Frkanecp>bp> ; Vesna Svetli膷i膰p>bp> ; Sr膽anka Tomi膰p>cp> ; Branka Vrane&scaron ; i膰p>ap> ; Ru啪a Frkanecp>ap>p> ; p>p>ruza.frkanec@gmail.comp>
• 关键词：Adamantane ; Mannose ; Liposome targeting ; AFM ; DLS
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：2012
• 期刊代码：14_00052736
• 类别：bio
• 出版时间：September, 2012
• 卷：1818
• 期：9
• 页码：2252-2259
• 文件大小：851 K

摘要

The aim of the present study was to encapsulate mannosylated 1-aminoadamantane and mannosylated adamantyltripeptides, namely [(2R)-N-(adamant-1-yl)-3-(伪,尾-d-mannopyranosyloxy)-2-methylpropanamide and (2R)-N-[3-(伪-d-mannopyranosyloxy)-2-methylpropanoyl]-d,l-(adamant-2-yl)glycyl-l-alanyl-d-isoglutamine] in liposomes. The characterization of liposomes, size and surface morphology was performed using dynamic light scattering (DLS) and atomic force microscopy (AFM). The results have revealed that the encapsulation of examined compounds changes the size and surface of liposomes. After the concanavalin A (ConA) was added to the liposome preparation, increase in liposome size and their aggregation has been observed. The enlargement of liposomes was ascribed to the specific binding of the ConA to the mannose present on the surface of the prepared liposomes. Thus, it has been shown that the adamantyl moiety from mannosylated 1-aminoadamantane and mannosylated adamantyltripeptides can be used as an anchor in the lipid bilayer for carbohydrate moiety exposed on the liposome surface.