Oxidant signaling for interleukin-13 gene expression in lung smooth muscle cells
- 作者：Geetanjali Bansal ; Chi-Ming Wong ; Lingling Liu ; Yuichiro J. Suzuki ; ys82@georgetown.edu
- 关键词：Cell growth ; Interleukin-13 ; Lung ; Reactive oxygen species ; Smooth muscle ; Free radicals
- 刊名：Free Radical Biology and Medicine
- 出版年：2012
- 期刊代码：105_08915849
- 类别：med
- 出版时间：1 May, 2012
- 卷：52
- 期：9
- 页码：1552-1559
- 文件大小：1101 K
摘要
The growth of airway and vascular smooth muscle cells occurs in various lung diseases including asthma, chronic obstructive pulmonary disease, bronchopulmonary dysplasia, lymphangioleiomyomatosis, and pulmonary hypertension. Although inflammatory responses are critical in these diseases, the relationship between smooth muscle cell growth and inflammatory mediators is poorly understood. This study demonstrates that platelet-derived growth factor (PDGF) promotes the expression of interleukin-13 (IL-13) in lung smooth muscle cells through an oxidant signaling mechanism. Treatment of cultured human airway or pulmonary vascular smooth muscle cells with PDGF promotes IL-13 mRNA and protein expression. IL-13 expression is also detected in smooth muscle of airways and pulmonary vessels in allergen-stimulated mice. PDGF activates the proximal 980-bp region of the IL-13 promoter. PDGF-induced IL-13 expression is suppressed by the inhibition of reactive oxygen species signaling such as by NAD(P)H oxidase inhibition, reactive oxygen species scavenging, and metal chelation. Treatment of cells with hydrogen peroxide at as low as 1 渭M also promotes IL-13 gene expression. PDGF-induced cell growth is suppressed by the neutralizing antibody against IL-13 as well as by reactive oxygen inhibitors, and recombinant IL-13 promotes the growth of airway smooth muscle cells. These results demonstrate that oxidant signaling activates IL-13 gene transcription in lung smooth muscle cells and that this signaling mechanism regulates cell growth.