Sustained outdoor H₂ production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor

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• 作者：Alessandra Adessi^a ; Giuseppe Torzillo^b ; Enrico Baccetti^a ; Roberto De Philippis^a ; ^c ; ^{roberto.dephilippis@unifi.it}
• 关键词：Outdoor hydrogen production ; Rhodopseudomonas palustris ; Tubular photobioreactors ; Bacteriochlorophyll a fluorescence ; Natural light/dark cycles
• 刊名：International Journal of Hydrogen Energy
• 出版年：2012
• 期刊代码：42_03603199
• 类别：ch
• 出版时间：May, 2012
• 卷：37
• 期：10
• 页码：8840-8849
• 文件大小：1008 K

摘要

Sunlight represents an important choice for low cost photosynthetic cultures irradiation, especially in up-scaled processes. The aim of this study was the assessment of the hydrogen production performances of Rhodopseudomonas palustris strain 42OL cultivated outdoors, under sunlight, in a 50 L tubular photobioreactor. Moreover, bacteriochlorophyll a fluorescence was used for the first time as a tool for monitoring the physiological status of Rp. palustris mass cultures.

The maximum H₂ production rate of 27.2 ml L^鈭? h^鈭?, and the mean rate per illuminated surface of 3.54 卤 1.53 L m^鈭? d^鈭? obtained resulted the highest so far reported for outdoor experiments carried out with purple bacteria in tubular photobioreactors of comparable dimensions. The in-situ measurement of bacteriochlorophyll a fluorescence (F_v/F_m) showed that the photosystem was unaffected by changing light irradiance during the day and remained fully active over the whole period of hydrogen production.