Hedistin: A novel antimicrobial peptide containing bromotryptophan constitutively expressed in the NK cells-like of the marine annelid, Nereis diversicolor
- 作者：Auré ; lie Tasiemski ; David Schikorski ; Franç ; oise Le Marrec-Croq ; Christelle Pontoire-Van Camp ; Cé ; line Boidin-Wichlacz ; Pierre-Eric Sautiè ; re
- 关键词：Bromotryptophan ; Antimicrobial peptide ; Annelids ; Invertebrate immunity ; NK cells like
- 刊名：Developmental and Comparative Immunology
- 出版年：2007
- 期刊代码：35_0145305x
- 类别：abs
- 出版时间：2007
- 卷：31
- 期：8
- 页码：749-762
- 文件大小：1218 K
摘要
A novel antimicrobial peptide, named hedistin was identified from the coelomocytes of Nereis diversicolor. Hedistin shows no obvious similarities with other known peptides and constitutes the first antimicrobial peptide containing bromotryptophans demonstrated in annelids. cDNA and mass spectrometry analysis revealed that, upon bacteria challenge, this peptide is secreted following processing of a precursor containing a signal peptide and prosequences. Hedistin was shown to possess an activity against a large spectrum of bacteria including the methicillin resistant Staphylococcus aureus and Vibrio alginolyticus. The gene was demonstrated to be constitutively and exclusively expressed in circulating NK cells like known to play an important role in the immunity of the sand worm. These data contrast with those observed in another annelid, the leech, in which genes coding for antimicrobial peptides are upregulated in a specific tissue and peptides are rapidly released into the hemolymph after septic injury.