Centrally acting leptin induces a resuscitating effect in haemorrhagic shock in rats

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• 作者：Jerzy Jochem^a ; ^{jjochem@poczta.onet.pl} ; Zbigniew Kalarus^b ; ^c ; Luca Spaccapelo^d ; Fabrizio Canalini^d ; Alessandra Ottani^d ; Daniela Giuliani^d ; Salvatore Guarini^d
• 关键词：leptin ; haemorrhagic shock ; sympathetic nervous system
• 刊名：Regulatory Peptides
• 出版年：2012
• 期刊代码：68_01670115
• 类别：neu
• 出版时间：10 June, 2012
• 卷：176
• 期：1-3
• 页码：45-50
• 文件大小：540 K

摘要

Centrally acting leptin induces the activation of the sympathetic nervous system with a pressor effect in normotensive rats. The purpose of the study was to examine central leptin-evoked action in critical haemorrhagic hypotension. In anaesthetized male Wistar rats subjected for irreversible haemorrhagic shock with mean arterial pressure (MAP) 20-25 mmHg haemodynamic parameters and plasma concentrations of adrenaline and noradrenaline were measured. Leptin given intracerebroventricularly (20 渭g) evoked long-lasting rises in MAP and heart rate (HR), with a subsequent increase in renal, mesenteric and hindquarters blood flows and a 100%survival at 2 h. MAP and peripheral blood flow changes were inhibited by a pre-treatment with 伪₁- and 伪₂-adrenoceptor antagonists prazosin (0.5 mg/kg) and yohimbine (1 mg/kg), while 尾-adrenoceptor antagonist propranolol (1 mg/kg) blocked leptin-induced HR changes, without influence on MAP, peripheral blood flows and survival. Twenty min after leptin treatment, there were higher plasma concentrations of noradrenaline, but not adrenaline, in comparison with the saline-treated control group. In conclusion, centrally acting leptin induces a long-lasting pressor effect with an improvement in the survival rate in haemorrhage-shocked rats. The effect may be associated with the activation of the sympathetic nervous system.