The antifungal effect of light emitting diode on Malassezia yeasts

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• 作者：Hyun Seung Wi ; Eui Young Na ; Sook Jung Yun ; Jee-Bum Lee ; ^{jbmlee@jnu.ac.kr} ; ^{jbmlee@chonnam.ac.kr}
• 关键词：Light emitting diode ; Malassezia species ; Antifungal effect ; Reactive oxygen species
• 刊名：Journal of Dermatological Science
• 出版年：2012
• 期刊代码：153_09231811
• 类别：med
• 出版时间：July, 2012
• 卷：67
• 期：1
• 页码：3-8
• 文件大小：867 K

摘要

Background

Malassezia (M.) species are members of the normal part of the skin flora, but they might induce or be involved with various cutaneous diseases. Although the role of Malassezia in the pathogenesis of cutaneous diseases is not fully understood, recent studies have shown that decreased density of Malassezia led to improvement of these diseases.

Objective

To identify the antifungal effect of light emitting diode (LED) against Malassezia, its antifungal mechanisms and the impact on the keratinocytes.

Methods

LED with various wavelengths (370-630 nm) on Malassezia furfur, Malassezia sympodialis and Malassezia globosa was irradiated according to dose and then the antifungal effects were thereafter assessed. After irradiating LED with 392.5 卤 1 nm of wavelength according to dose on Malassezia species, reactive oxygen species (ROS) and lipid hydroperoxide production assay were measured. In addition, cell viability and inflammatory cytokines (IL-1伪, IL-1尾, TNF-伪, TGF-尾, TLR-2 and COX-2) expressions in normal human epidermal keratinocytes (NHEKs) by LED irradiation were evaluated.

Results

The growth of Malassezia species was dose-dependently suppressed by both LED with 380 卤 2 and 392.5 卤 1 nm wavelengths. The increases of intracellular and extracellular ROS by LED irradiation with 392.5 卤 1 nm wavelengths were significantly observed compared to control group. The cell viability and cytokines in NHEKs were not significantly affected by LED irradiation under 5 J/cm² in vitro.

Conclusion

LED irradiation with 380 卤 2 and 392.5 卤 1 nm wavelengths proved to have antifungal effect against Malassezia species and no impact on NHEKs under 5 J/cm². The findings suggest that LED might be an adjunctive therapeutic light tool against Malassezia yeasts related cutaneous diseases.