Enzyme membrane reactor in isolation of antioxidative peptides from oil industry waste: A comparison with non-peptidic antioxidants

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• 作者：Ranjana Das^a ; ^{ranjanads@yahoo.co.in} ; Santinath Ghosh^b ; Chiranjib Bhattacharjee^a
• 关键词：Seed meal ; Protein hydrolysate ; Peptide antioxidant ; Enzyme membrane reactor ; Ultrafiltration
• 刊名：Lebensmittel-Wissenschaft und -Technologie
• 出版年：2012
• 期刊代码：121_00236438
• 类别：abs
• 出版时间：July, 2012
• 卷：47
• 期：2
• 页码：238-245
• 文件大小：1038 K

摘要

Edible oil industries suffer from the problem of seed meal utilization, which is recognized as a byproduct of edible oil. Present work has investigated production of peptide antioxidants, from oil seed meals to meet the increasing crave for natural antioxidants in food and pharmaceutical industries. Metallo-endopeptidase 鈥楶rotease A Amano 2G鈥?(Aspergillus oryzae) was used to hydrolyze seed protein isolate in enzyme membrane reactor (EMR) and protein hydrolysate was sequentially fractionated by ultrafiltration to obtain potential peptide fraction. Degree of hydrolysis was varied within enzyme to substrate ratio 0.1-2聽g/100聽g and hydrolysis time 10-60聽min to maximize peptide yield and antioxidant activity of peptides in聽vitro. Controlled hydrolysis with enzyme dose of 2聽g/100聽g, exhibited peptide yield of 4.60聽卤聽0.08聽mg/100聽mg meal protein in membrane reactor (DH 30.7%) and 4.23聽卤聽0.22聽mg/100聽mg meal protein in batch mode of hydrolysis (DH 29.3%). Antioxidant potential of peptide fractions were compared with commercial non-peptidic antioxidants and major findings confirm superior activity for protein fragments (IC₅₀-0.062聽mg/mL) as compared to protein isolate (IC₅₀-0.038聽mg/mL), with enhanced product stability by preventing oxidative deterioration. Results substantiate the prospect of EMR in obtaining peptides from edible oil industry waste, having comparable antioxidative potential with commercial non-peptidic antioxidants.