Establishing the phenotype in novel acute and chronic murine models of allergic asthma
- 作者：Sofia Fern ; ez-Rodriguez ; William R. Ford ; Kenneth J. Broadley ; Emma J. Kidd
- 关键词：Allergic asthma ; Mouse ; Acute and chronic models ; In vivo ; Lung function
- 刊名：International Immunopharmacology
- 出版年：2008
- 期刊代码：123_15675769
- 类别：med
- 出版时间：May 2008
- 卷：8
- 期：5
- 页码：756-763
- 文件大小：467 K
摘要
Allergic asthma is a chronic disease of the airways, with superimposed acute inflammatory episodes which correspond to exacerbations of asthma. Two novel models of allergic asthma have been developed in mice receiving the same allergen sensitisation, but with acute or chronic allergen exposures, the latter to mimic the human situation more closely. Ovalbumin-sensitised mice were challenged by ovalbumin inhalation twice on the same day for the acute model, and 18 times over a period of 6 weeks for the chronic model. Lung function was monitored in conscious, unrestrained mice immediately after the last challenge for up to 12 h. Airway responsiveness to inhaled methacholine and serum antibody levels were determined 24 h after challenge. Bronchoalveolar inflammatory cell recruitment was determined at 2 or 24 h.