Combined astaxanthin and fish oil supplementation improves glutathione-based redox balance in rat plasma and neutrophils

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• 作者：Marcelo Paes Barros^a ; Douglas Popp Marin^b ; Anaysa Paola Bolin^b ; Rita de C&#225 ; ssia Santos Macedo^b ; Thais Regina Campoio^b ; Claudio Fineto Jr.^b ; Beatriz Alves Guerra^b ; Tatiana Geraldo Polotow^a ; Cristina Vardaris^a ; Rita Mattei^c ; Rosemari Otton^b ; ^{rosemari.otton@cruzeirodosul.edu.br}
• 关键词：DHA ; EPA ; Omega-3 ; Carotenoid ; Anti-inflammatory ; Leukocyte
• 刊名：Chemico-Biological Interactions
• 出版年：2012
• 期刊代码：6_00092797
• 类别：pha
• 出版时间：15 April, 2012
• 卷：197
• 期：1
• 页码：58-67
• 文件大小：450 K

摘要

The present study aimed to investigate the effects of daily (45 days) intake of fish oil (FO; 10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) and/or natural ASTA (1 mg ASTA/kg BW) on oxidative stress and functional indexes of neutrophils isolated from Wistar rats by monitoring superoxide (), hydrogen peroxide (H₂O₂), and nitric oxide (NO^{) production compared to the progression of auto-induced lipid peroxidation and Ca2+ release in activated neutrophils. Furthermore, phagocytic capacity, antioxidant enzyme activities, glutathione-recycling system, and biomarkers of lipid and protein oxidation in neutrophils were compared to the redox status. Our results show evidence of the beneficial effects of FO + ASTA supplementation for immune competence based on the redox balance in plasma (significant increase in GSH-dependent reducing power), non-activated neutrophils (increased activity of the glutathione-recycling enzymes GPx and GR) and PMA-activated neutrophils (lower , H₂O₂, and NO generation, reduced membrane oxidation, but higher phagocytic activity). Combined application of ASTA and FO promoted hypolipidemic/hypocholesterolemic effects in plasma and resulted in increased phagocytic activity of activated neutrophils when compared with ASTA or FO applied alone. In PMA-activated neutrophils, ASTA was superior to FO in exerting antioxidant effects. The bulk of data reinforces the hypothesis that habitual consumption of marine fish (e.g. salmon, which is a natural source of both astaxanthin and fish oil) is beneficial to human health, in particular by improving immune response and lowering the risk of vascular and infectious diseases.}