Isolation of brefeldin A from Eupenicillium brefeldianum broth using macroporous resin adsorption chromatography

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• 作者：Ya-Jun Wang^a ; ^b ; Ye-Fei Wu^a ; ^b ; Feng Xue^a ; ^b ; Zhi-Xian Wu^a ; ^b ; Ya-Ping Xue^a ; ^b ; Yu-Guo Zheng^a ; ^b ; ^{zhengyg@zjut.edu.cn} ; Yin-Chu Shen^a ; ^b
• 关键词：Brefeldin A ; Macroporous resin ; Purification ; Adsorption chromatography ; Crystallization
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：1 May, 2012
• 卷：895-896
• 期：Complete
• 页码：146-153
• 文件大小：998 K

摘要

Brefeldin A (BFA) is a macrolide lactone antibiotic, possessing antitumor, antiviral, antifungal activities. In this work, a separation strategy involving one-step macroporous resin adsorption chromatography combined with crystallization was established for BFA purification from Eupenicillium brefeldianum CCTCC M 208113 fermentation broth. Among six macroporous resin adsorbents tested, the non-polar resin HZ830 had the best adsorption and desorption performance. The static equilibrium adsorption data fitted well with the Freundlich equation, and the adsorption kinetic followed the pseudo-second order model. Through experimental optimization of column adsorption and desorption, BFA in purity of 90.4%(w/w), 92.1%(w/w) yield was obtained by a one-step macroporous resin adsorption chromatography, using a stepwise elution protocol. Furthermore, high purity (>99%, w/w) of BFA crystals were prepared from E. brefeldianum CCTCC M 208113 fermentation broth in an overall recovery of 67.0%(w/w), using a combination of adsorption chromatography packed with non-polar macroporous adsorbent HZ830 and crystallization in acetone.