siRNA carriers based on carbosilane dendrimers affect zeta potential and size of phospholipid vesicles

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• 作者：Maksim Ionov^a ; ^{maksion@biol.uni.lodz.pl} ; Zuzana Garaiova^b ; Iveta Waczulikova^b ; Dominika Wró ; bel^a ; El偶bieta P臋dziwiatr-Werbicka^a ; Rafael Gomez-Ramirez^c ; Francisco Javier de la Mata^c ; Barbara Klajnert^a ; Tibor Hianik^b ; Maria Bryszewska^a
• 关键词：PBS ; phosphate-buffered saline ; CBD ; carbosilane dendrimers ; DMPC ; 1 ; 2-dimyristoyl-sn-glycero-3-phosphocholine ; DPPG ; dipalmitoylphosphatidylglycerol ; LUVs ; large unilamellar vesicles
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：2012
• 期刊代码：14_00052736
• 类别：bio
• 出版时间：September, 2012
• 卷：1818
• 期：9
• 页码：2209-2216
• 文件大小：1063 K

摘要

One of the major limitations in gene therapy is an inability of naked siRNA to passively diffuse through negatively charged cell membranes. Therefore, the siRNA transport into a cell requires efficient carriers. In this work we analyzed the charge-dependent interaction of the complexes of cationic carbosilane dendrimers (CBD) and anti-HIV siRNA (dendriplexes) with the model membranes 鈥?large unilamellar vesicles (LUV). We used the second generation of branched with CBD carbon-silicon bonds (CBD-CS) which are water-stable and that of oxygen-silicon bonds (CBD-OS) which are slowly hydrolyzed in aqueous solutions. The LUVs were composed of zwitterionic dimyristoylphosphatidylcholine (DMPC), negatively charged dipalmitoylphosphatidylglycerol (DPPG) and their mixture (DMPC/DPPG, molar ratio 7:3). The interaction of dendriplexes with LUVs affected both zeta potential and size of the vesicles. The changes of these values were larger for the negatively charged LUV. CBD-CS resulted in the decrease of zeta potential values to more negative ones, whereas an opposite effect took place for CBD-OS suggesting a different kind of interaction between LUVs and the dendriplexes. The results indicate that both CBD-CS and CBD-OS can be used for transport of siRNA into the cells. However, CBD-CS are preferred due to a better stability in water and improved bioavailability of siRNA on their surface.