The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles

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• 作者：Emilio I. Alarcon^a ; ¹ ; Klas Udekwu^b ; ¹ ; Må ; rten Skog^c ; Natalia L. Pacioni^a ; Kevin G. Stamplecoskie^a ; Marí ; a Gonzá ; lez-Bé ; jar^a ; Naresh Polisetti^c ; Abeni Wickham^c ; Agneta Richter-Dahlfors^b ; ; May Griffith^c ; ^d ; ; Juan C. Scaiano^a ; ^c ; ^{tito@photo.chem.uottawa.ca} ; ^{titoscaiano@mac.com}
• 关键词：Silver nano particles ; Type-I collagen ; Poly-L-Lysine ; Cell toxicity ; Antimicrobial activity
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：June, 2012
• 卷：33
• 期：19
• 页码：4947-4956
• 文件大小：1889 K

摘要

Spherical 3.5聽nm diameter silver nanoparticles (AgNP) stabilized in type I collagen () were prepared in minutes (5-15聽min) at room temperature by a photochemical method initiated by UVA irradiation of a water-soluble non-toxic benzoin. This biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. Thus, while keratinocytes and fibroblasts were not affected by , it was bactericidal against Bacillus megaterium and E.聽coli but only bacteriostatic against S.聽epidermidis. In particular, the bactericidal properties displayed by were proven to be due to AgNP in , rather than to released silver ions, since equimolar concentrations of Ag are about four times less active than based on total Ag content. This new biocomposite was stable over a remarkable range of NaCl, phosphate, and 2-(N-morpholino)ethanesulfonic acid concentrations and for over one month at 4聽掳C. Circular dichroism studies show that the conformation of collagen in remains intact. Finally, we have compared the properties of with a similar biocomposite prepared using 伪-poly-l-Lysine and also with citrate聽stabilized AgNP; neither of these materials showed comparable biocompatibility, stability, or anti-bacterial activity.