Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine)
- 作者：Seifert ; Karin ; Matu ; Sangeeta ; Javier Pé ; rez-Victoria ; F. ; Castanys ; Santiago ; Gamarro ; Francisco ; et. al.
- 关键词：Leishmania ; Hexadecylphosphocholine ; Miltefosine ; Drug resistance
- 刊名：International Journal of Antimicrobial Agents
- 出版年：2003
- 期刊代码：126_09248579
- 类别：med
- 出版时间：October, 2003
- 卷：22
- 期：4
- 页码：380-387
- 文件大小：259 K
摘要
Leishmania donovani promastigote lines resistant to hexadecylphosphocholine (HePC, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 μM were developed in vitro by continuous step-wise drug pressure. The 40 μM line was 15 times more resistant to HePC than the wild-type clone and showed cross-resistance to the ether lipid ET-18-OCH3 (edelfosine) but not to the standard anti-leishmanial drugs. Resistance was stable up to 12 weeks in drug-free culture medium. No amplification of specific genes, including the multidrug resistance P-glycoprotein gene, could be detected in the resistant parasites.