Posttransplant hematopoiesis in patients undergoing sibling allogeneic stem cell transplantation reflects that of their respective donors although with a lower functional capability

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• 作者：Fermin M. Sanchez-Guijo ; Luis-Ignacio Sanchez-Abarca ; Eva Villaron ; Natalia Lopez-Holgado ; Mercedes Alberca ; Lourdes Vazquez ; Jose A. Perez-Simon ; Jesus Lopez-Fidalgo ; Alberto Orfao ; Maria-Dolores Cab
• 刊名：Experimental Hematology
• 出版年：2005
• 期刊代码：100_0301472x
• 类别：med
• 出版时间：2005
• 卷：33
• 期：8
• 页码：935-943
• 文件大小：328 K

摘要

We tested the principle of whether patient long-term hematopoiesis following allogeneic stem cell transplantation (allo-SCT) reflects the characteristics of the hematopoiesis of their respective donor. For this purpose, we analyzed bone marrow (BM) hematopoiesis using long-term cultures (LTC), delta assays, and clonogeneic assays as well as CD34⁺ cells and their subsets by flow cytometry in a series of 37 patients undergoing allo-SCT, and we compared it to that of their respective human leukocyte antigen–matched sibling donors in a paired study performed more than 1 year after the transplant procedure. Interestingly, the main factor that influenced post-allo-SCT BM hematopoiesis in the long term was donor hematopoiesis. Nevertheless, compared to their respective donors, patients exhibited a significantly lower number of colony-forming units granulomonocytic, burst-forming units erythroid, and immature progenitors (CD34⁺⁺/CD38^dim/CD90⁺/CD133⁺ cells, LTC-initiating cells, and colonies generated in the delta assay). Moreover, BM stromal function was diminished in patients undergoing allo-SCT compared to their donors. In addition, the presence of chronic graft-versus-host disease under immunosuppressive treatment also conditioned an impaired hematopoietic function. In summary, our study shows that BM hematopoiesis evaluated more than 1 year after an allo-SCT mainly reproduces that of their respective donors, although with a significantly decreased in vitro activity.