The importance of bystander effects in radiation therapy in melanoma skin-cancer cells and umbilical-cord stromal stem cells

详细信息	查看全文 | 推荐本文 |

• 作者：Jaime Gó ; mez-Millá ; n^b ; ¹ ; Iana Suly Santos Katz^c ; ¹ ; Virgí ; nea de Araujo Farias^a ; Jose-Luis Linares-Ferná ; ndez^a ; Jesú ; s Ló ; pez-Peñ ; alver^a ; Gustavo Ortiz-Ferró ; n^a ; Carmen Ruiz-Ruiz^a ; Francisco Javier Oliver^d ; José ; Mariano Ruiz de Almodó ; var^a ; ^e ; ^{jmrdar@ugr.es}
• 关键词：Melanoma cancer cells ; Umbilical-cord stromal stem cells ; Radiosensitivity ; Radiation-induced bystander cell-death ; Fractionation in the bystander effect
• 刊名：Radiotherapy and Oncology
• 出版年：2012
• 期刊代码：231_01678140
• 类别：med
• 出版时间：March, 2012
• 卷：102
• 期：3
• 页码：450-458
• 文件大小：507 K

摘要

Purpose

To examine direct and bystander radiation-induced effects in normal umbilical-cord stromal stem cell (HCSSC) lines and in human cancer cells.

Materials and methods

The UCSSC lines used in this study were obtained in our laboratory. Two cell lines (UCSSC 35 and UCSSC 37) and two human melanoma skin-cancer cells (A375 and G361) were exposed to ionizing radiation to measure acute radiation-dosage cell-survival curves and radiation-induced bystander cell-death response.

Normal cells, although extremely sensitive to ionizing radiation, were resistant to the bystander effect whilst tumor cells were sensitive to irradiated cell-conditioned media, showing a dose-response relationship that became saturated at relatively low doses.

We applied a biophysical model to describe bystander cell-death through the binding of a ligand to the cells. This model allowed us to calculate the maximum cell death (蠂_max) produced by the bystander effect together with its association constant (K_By) in terms of dose equivalence (Gy). The values obtained for K_By in A375 and G361 cells were 0.23 and 0.29 Gy, respectively.

Conclusion

Our findings help to understand how anticancer therapy could have an additional decisive effect in that the response of sub-lethally hit tumor cells to damage might be required for therapy to be successful because the survival of cells communicating with irradiated cells is reduced.