Control of hypertension in rats using volatile components of leaves of Taxus chinensis var. mairei

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• 作者：Wei-Xia Yang^a ; ^{snywx@nwsuaf.edu.cn} ; Zhang-Guang Zhao^b ; Lai-Hong Wang^b ; Shan-Jiang Yu^a ; Zong-Suo Liang^a ; ^{liangzs@ms.iswc.ac.cn}
• 关键词：Hypertensive rats ; VCLT ; SBP ; Plasma NO ; Plasma angiotensin II
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：7 May, 2012
• 卷：141
• 期：1
• 页码：309-313
• 文件大小：450 K

摘要

Ethnopharmacological relevance

The leaves of Taxus chinensis var. mairei (Taxaceae) is used traditionally to fill pillows in some rural areas of China. Its volatile substances have been speculated to be capable of improving sleep quality, making blood pressure stable, and having diuretic capacity as recorded in Ancient Chinese Materia Medica. Using animal models and new technologies, we confirmed the hypotensive potential of volatile components from leaves of Taxus chinensis var. mairei (VCLT).

Materials and methods

VCLT was obtained by supercritical CO₂ extraction equipment from Taxus chinensis var. mairei fresh leaves. Hypertensive rats were pre-induced by intraperitoneal (i,p.) injection of N蠅-Nitro-l-Ariginine (l-NNA) for 15 days (15 mg/kg, twice a day), then divided into 5 groups and subjected to the following treatments. l-NNA group (group 1) receiving l-NNA alone (15 mg/kg, i.p., twice per day for 6 weeks); in addition to receiving l-NNA same as group 1, Hydrochlorothiazide (HDZ) group (group 2) receiving HDZ (orally administration, 5 mg/kg, once per day for 6 weeks); VCLT groups (groups 3-5), including VCLT1, VCLT2, VCLT3. The VCLT rats were housed in an enclosed cage (2 rats/0.064 m³). VCLT was mixed well and sprayed on fresh leaves surface of Taxus chinensis var. mairei (100 ml/kg) with three dosages: 167 g/kg (VCLT1), 233 g/kg (VCLT2) and 333 g/kg (VCLT3), respectively. Systolic Blood Pressure (SBP), plasma nitric oxide (NO), plasma angiotensin II, postprandial blood glucose, fasting blood glucose and blood lipids were determined.

Results

VCLT prevented the increase of SBP and plasma angiotensin II in l-NNA treated rats. Although VCLT does not significantly reduce blood triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C), it decreases total cholesterol (TC) while increasing plasma NO levels in a dose-dependent manner.

Conclusion

VCLT can be used as a natural and supplementary reagents for the treatment of hypertension.