Immuno-enhancing activity of sulfated Auricularia auricula polysaccharides

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• 作者：The Luong Nguyen^a ; ¹ ; Deyun Wang^a ; ¹ ; Yuanliang Hu^a ; ^{ylhu@njau.edu.cn} ; Yunpeng Fan^a ; Junmin Wang^a ; Saifuding Abula^a ; Liwei Guo^a ; Jing Zhang^a ; Shem Kakai khakame^b ; Bao Khanh Dang^c
• 关键词：AAP ; Auricularia auricula polysaccharide ; sAAP ; sulfated AAP ; CEF ; chicken embryo fibroblast ; ConA ; concanavalin A ; CSA ; chlorosulfonic acid ; Pyr ; pyridine ; HI ; hemagglutination inhibition ; PHA ; phytohemagglutinin ; NDV ; Newcastle disease virus ; ND ; Newcas
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 August, 2012
• 卷：89
• 期：4
• 页码：1117-1122
• 文件大小：409 K

摘要

The crude total Auricularia auricula polysaccharide (AAP_ct) was extracted by water decoction and ethanol precipitation, protein was removed to obtain total A. auricula polysaccharide (AAP_t), then was graded into AAP₁ and AAP₂ through column chromatography. sAAP_t, sAAP₁ and sAAP₂ were prepared by chlorosulfonic acid-pyridine method. In vitro test, the effects of sAAP_t, sAAP₁, sAAP₂, AAP_t, AAP₁ and AAP₂, on chicken peripheral lymphocytes proliferation were compared. The results showed that sAAP_t and sAAP₁ demonstrated better effect. In vivo test, 14-day-old chickens were injected respectively with sAAP_t, sAAP₁, AAP_t and AAP₁ at the first vaccination of ND vaccine, once a day for three days. On days 7, 14, 21 and 28 after the first vaccination, the peripheral lymphocytes proliferation and antibody titer were determined. The results indicated that sAAP_t possessed the best efficacy and would be expected to be used as a component of a new-type immunopotentiator.