Rev-erb伪 regulates circadian rhythms and StAR expression in rat granulosa cells as identified by the agonist GSK4112

详细信息	查看全文 | 推荐本文 |

• 作者：Huatao Chen^a ; Guiyan Chu^a ; ¹ ; Lijia Zhao^a ; Nobuhiko Yamauchi^a ; Yasufumi Shigeyoshi^b ; Seiichi Hashimoto^c ; Masa-aki Hattori^a ; ^{mhattori@agr.kyushu-u.ac.jp}
• 关键词：Rev-erb伪 ; Circadian rhythm ; StAR ; GSK4112 ; Granulosa cell
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：6 April, 2012
• 卷：420
• 期：2
• 页码：374-379
• 文件大小：492 K

摘要

The Rev-erb伪 gene is regarded as a circadian clock gene and clock-regulated gene which regulates the circadian transcriptional/translational loop in a subtle way. Here, we first detected the circadian oscillation in mature granulosa cells from antral follicles using a real-time monitoring system of Per2 promoter activity with the addition of FSH. Then we used GSK4112, an agonist ligand of Rev-erb伪, to investigate the function of Rev-erb伪. GSK4112 treatment significantly reduced the Per2-dLuc amplitude and induced the Per2 oscillation phase advance shift. GSK4112 significantly inhibited Bmal1 mRNA expression, whereas it did clearly stimulate expression of StAR mRNA in a dose-dependent manner. Our data are the first to show the Rev-erb伪 function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erb伪 may coordinate circadian rhythm and metabolism in rat ovaries.