- 作者：Yi-Chun Chou ; M.Sc.a ; b ; Hui-Min Su ; Ph.D.c ; Tzu-Wen Lai ; M.Sc.b ; Jong-Ho Chyuan ; M.Sc.d ; Pei-Min Chao ; Ph.D.b ; pmchao@mail.cmu.edu.tw
- 关键词：Cis-9 ; trans-11 ; trans-13 onjugated linolenic acid ; Linolenic acid ; Adipogenesis ; 3T3-L1 ; ERK/MAPK
- 刊名：Nutrition
- 出版年：2012
- 期刊代码：81_08999007
- 类别：abs
- 出版时间：July-August, 2012
- 卷：28
- 期：7-8
- 页码：803-811
- 文件大小：630 K
Objective
The aim of this study was to investigate the adipogenic effect of cis-9, trans-11, trans-13-conjugated linolenic acid (c9,t11,t13-CLN), a fatty acid naturally present in bitter melon.Methods
The 3T3-L1 murine preadipocyte cell line was used to test the effect of saponifiables from whole bitter melon and of commercially prepared pure c9,t11,t13-CLN on adipocyte differentiation. The effect of c9,t11,t13-CLN on 3T3-L1 cell viability was also tested at proliferation, mitotic clonal expansion, and terminal differentiation stages.
Results
Compared to the free fatty acid control mixture, the proadipogenic effect on 3T3-L1 was less potent using saponifiables obtained from bitter melon. C9,t11,t13-CLN, unlike its non-conjugated counterpart linolenic acid (LN) or other common fatty acids such as oleic acid or linoleic acid, exerted no proadipogenic effect on 3T3-L1. In contrast to LN displaying no cytotoxic effect at a concentration 鈮?00 渭M, c9,t11,t13-CLN caused a dose-dependent reduction in the viability of pre- and postconfluent preadipocytes associated with apoptosis. Sustained ERK/MAPK activation, accompanied by increased peroxisome proliferator-activated receptor 纬 phosphorylation, was seen in c9,t11,t13-CLN-treated cells at initiation of differentiation.
Conclusion
C9,t11,t13-CLN is less adipogenic for 3T3-L1 cells than LN and this is partly due to its apoptotic effect on proliferating preadipocytes and to the sustained ERK phosphorylation seen during mitotic clonal expansion.