8-17 DNAzyme modified with purine analogs in its catalytic core: The conservation of the five-membered moieties of purine residues

详细信息	查看全文 | 推荐本文 |

• 作者：Wei Rong^a ; Liang Xu^b ; Yang Liu^b ; Jianping Yu^a ; ^{yujp666666@163.com} ; Ying Zhou^a ; Keliang Liu^b ; Junlin He^b ; ^{hejunlin@yahoo.com}
• 关键词：8&ndash ; 17 DNAzyme ; Purine analogs ; Catalytic cleavage ; Chemical modification ; 7-Nitrogen atom
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 July, 2012
• 卷：22
• 期：13
• 页码：4238-4241
• 文件大小：282 K

摘要

8-17 DNAzyme is characterized by its recurrence in different in vitro selections and versatile cleavage sites, leading to extensive studies on its structural properties and applications. We evaluated the purine residues (A6, G7, G11, A12, G14, and A15) in the catalytic core of 8-17 DNAzyme of their five-membered ring moiety with purine analogs 1-5 to have an insight into the conservation of the residues at the level of functional groups. The 7-nitrogen atom in the AGC loop was demonstrated to be strictly conserved for the cleavage reaction. But such modifications exerted favorable effect at G11 of the base-pair stem and A12 in the single-strand loop, directing toward more efficient DNAzymes. Even the most conserved G14 could tolerate such modifications. These results demonstrated that chemical modification on the functional groups is a feasible approach to gain an insight into the structural requirement in the catalytic reaction of DNAzymes. It also provided modification sites for introduction of signaling molecules used for mechanistic and folding studies of 8-17 DNAzyme.