Establishment of a pair of novel cloned tumour cell lines with or without metastatic potential from canine mammary adenocarcinoma
- 作者：K. Murai ; T. Nakagawa ; anakaga@mail.ecc.u-tokyo.ac.jp ; Y. Endo ; A. Kamida ; K. Yoshida ; M. Mochizuki ; R. Nishimura ; N. Sasaki
- 关键词：Mammary adenocarcinoma ; Caine ; Cloned cell line ; Characterization ; Xenograft ; Metastasis
- 刊名：Research in Veterinary Science
- 出版年：2012
- 期刊代码：272_00345288
- 类别：med
- 出版时间：August, 2012
- 卷：93
- 期：1
- 页码：468-472
- 文件大小：1820 K
摘要
We produced 23 cloned cell lines from parental CHMp, which was previously established from a canine mammary adenocarcinoma patient in our laboratory. Two representative cloned cell lines, namely, CHMp-5b and -13a, were selected and characterized for cellular morphology, growth potential and expression of some tumour-related proteins. Subsequently, we transplanted the 2 tumour cell lines orthotopically into female nude mice to examine their tumorigenicity and metastatic potential. Interestingly, despite sharing the same origin, only CHMp-5b cells metastasized to the lung. Our results indicate that a comparison between these 2 cell lines at the molecular level will help us understand mechanisms of tumour progression, especially in the context of distant metastases originating from canine mammary gland tumours.