Partition and recovery of phytase from Absidia blakesleeana URM5604 using PEG-citrate aqueous two-phase systems

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• 作者：Maria Luiza Carvalho Neves^a ; ^b ; ^c ; Tatiana Souza Porto^b ; ^d ; Cristina Maria Souza-Motta^e ; Michele Rigon Spier^f ; Carlos Ricardo Soccol^f ; Keila Aparecida Moreira^b ; ^d ; Ana Lú ; cia Figueiredo Porto^a ; ^b ; ^{analuporto@yahoo.com.br}
• 关键词：Aqueous two-phase system ; Phytase ; Partition ; Recovery ; PEG/citrate
• 刊名：Fluid Phase Equilibria
• 出版年：2012
• 期刊代码：32_03783812
• 类别：ce
• 出版时间：25 March, 2012
• 卷：318
• 期：Complete
• 页码：34-39
• 文件大小：188 K

摘要

The partition and recovery behavior of phytase, produced by solid-state cultivation utilizing citrus pulp as substrate, was determined in an aqueous two-phase system (ATPS), composed by polyethylene glycol (PEG-citrate). The 2⁴ factorial design was conducted to evaluate the effect of the following independent variables: PEG molar mass, PEG and citrate concentrations and pH, while partition coefficient, recovery and purification factor were selected as the variables responses. The best recovery in phytase activity (115.24%) condition was obtained with 26%(w/w) PEG 8000 (g/mol), 12%(w/w) sodium citrate at pH 8.0, showing a purification factor of 1.35. However, the highest partition coefficient (14.42) was observed within a 26%(w/w) PEG 400 (g/mol) and a 20%(w/w) sodium citrate at pH 6.0. The independent variables which more influenced on the partition coefficient and recovery were citrate concentration and PEG mass molar, respectively. The results suggest that PEG-citrate ATPS is an interesting and efficient alternative to partition and recovery of phytase from fermentation broth produced by Absidia blakesleeana URM5604.