Mitigation by vitamin C of the genotoxic effects of nicotine in mice, assessed by the comet assay and micronucleus induction

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• 作者：Vivian F.S. Kahl ; Juliana M. Reyes ; Merielen S. Sarmento ; Juliana da Silva ; ^{juliana.silva@ulbra.br} ; ^{julianadasilva@uol.com.br}
• 关键词：Nicotine ; Vitamin C ; Comet assay ; Antigenotoxicity ; Micronucleus test
• 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
• 出版年：2012
• 期刊代码：31_13835718
• 类别：pha
• 出版时间：15 May, 2012
• 卷：744
• 期：2
• 页码：140-144
• 文件大小：242 K

摘要

Nicotine has been reported to cause acute toxicity and to present long-term risks, such as chromosomal damage and genetic instability. The genotoxicity of nicotine may be mediated partly by an oxidative mechanism. We have evaluated the effects of the antioxidant vitamin C on nicotine-induced genotoxicity in mice. The comet assay and the micronucleus test were used to assess the effects of nicotine (15 mg/kg) at different exposure times (2, 4, and 24 h in the comet assay; 24 h in the micronucleus test). Pretreatment with vitamin C 24 h before nicotine exposure strongly protected mice against nicotine-induced DNA damage.