Fed-batch cultivation of Wautersia eutropha

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• 作者：Pallavi Patwardhan ; Ashok K. Srivastava
• 关键词：PHB ; Modeling ; Fed-batch cultivation ; Wautersia eutropha ; Biopolymer
• 刊名：Bioresource Technology
• 出版年：2008
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：April 2008
• 卷：99
• 期：6
• 页码：1787-1792
• 文件大小：142 K

摘要

Batch kinetics of polyhydroxybutyrate (PHB) synthesis in a bioreactor under controlled conditions of pH and dissolved oxygen gave a biomass of 14 g l⁻¹ with a PHB concentration of 6.1 g l⁻¹ in 60 h. The data of the batch kinetics was used to develop a mathematical model, which was then extrapolated to fed-batch by incorporating the dilution due to substrate feeding. Offline computer simulation of the fed-batch model was done to develop the nutrient feeding strategies in the fed-batch cultivation. Fed-batch strategies with constant feeding of only nitrogen and constant feeding of both nitrogen and fructose were tried. Constant feeding strategy for nitrogen and fructose gave a better PHB production rate of 0.56 g h⁻¹ over the value obtained in batch cultivation (PHB production rate – 0.4 g h⁻¹).