Fed-batch fermentation and supercritical fluid extraction of heterotrophic microalgal Chlorella protothecoides lipids

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• 作者：Yen-Hui Chen ^{ychen@clemson.edu} ; Terry H. Walker ; ^{walker4@clemson.edu}
• 关键词：Chlorella protothecoides ; Fed-batch fermentation ; Heterotrophic growth ; Lipids ; Supercritical carbon dioxide extraction
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：512-517
• 文件大小：397 K

摘要

Lipids obtained from Chlorella protothecoides in heterotrophic cultivation are considered a suitable feedstock for biodiesel production. In this study, glucose fed-batch fermentation was performed to increase final biomass and lipid production. The biomass productivity and lipid productivity were 6.28 and 2.06 g/L day, respectively. Biomass/glucose conversion and the lipid/glucose conversion were 43.3%and 14.2%, respectively. Extraction of lipids from algae has been identified as a key bottleneck in bioprocessing operations. Supercritical carbon dioxide (SC-CO₂) was applied for neutral lipids extraction and the SC-CO₂ kinetics was investigated by the Goto et al. model. The modeling showed a good fit with experimental data. Additionally, neutral lipids extracted by SC-CO₂ displayed a suitable fatty acid profile for biodiesel [mainly C18:1 (60.0%), C18:2 (18.7%) and C16:0 (11.5%)]. Our study demonstrated the ability to produce high levels of neutral lipids through heterotrophic algal culture and subsequent extraction of lipids with SC-CO₂ method developed.