Rapid on-site detection of Acidovorax citrulli by cross-priming amplification

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• 作者：Jing Zhang^a ; Qian Tian^b ; Shui-fang Zhu^b ; Wen-jun Zhao^b ; ^{wenjunzhao@188.com} ; Feng-quan Liu^a ;
• 关键词：Acidovorax citrulli ; Cross-priming amplification ; Detection
• 刊名：Molecular and Cellular Probes
• 出版年：2012
• 期刊代码：104_08908508
• 类别：imm
• 出版时间：August, 2012
• 卷：26
• 期：4
• 页码：175-176
• 文件大小：204 K

摘要

Cross-priming amplification (CPA) for Acidovorax citrulli detection was evaluated in this study. The sensitivity of CPA assay for pure bacterial culture was 3.7聽脳聽10³聽CFU/ml. Bacteria on naturally infected watermelon seeds were detected using CPA assay, suggesting this method is suitable for A.聽citrulli on-site detection from watermelon seeds.