Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Bras铆lia, Federal District, Brazil

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• 作者：Daniella de Sousa Mendes Moreira Alves^a ; ^{alveslm@gmail.com} ; Aline Silva Moraes^a ; Nadjar Nitz^b ; Mayara Gabriele Carvalho de Oliveira^a ; Mariana Machado Hecht^b ; Rodrigo Gurgel-Gonç ; alves^a ; Cé ; sar Augusto Cuba Cuba^a ; ^c
• 关键词：Acanthamoeba ; Soil ; Swimming pool ; Water feature ; Genotypes
• 刊名：Experimental Parasitology
• 出版年：2012
• 期刊代码：99_00144894
• 类别：imm
• 出版时间：June, 2012
• 卷：131
• 期：2
• 页码：239-244
• 文件大小：223 K

摘要

Species of Acanthamoeba can cause keratitis and brain infections. The characterization of environmental isolates is necessary to analyze the risk of human infection. We aimed at identifying and genotyping Acanthamoeba isolates from soil, swimming pools, and water features in Bras铆lia, Federal District, Brazil, as well as determining their physiological characteristics and pathogenic potential. Among the 18 isolates studied, eight were similar to genotype T5, five to T4, and one to T2/T6, classified by the sequence analysis of 18S rDNA. Genotypes of four isolates were not determined. Ten isolates (55%) grew at 37 掳C and seven (39%) grew in media with 1.5 M mannitol, which are the physiological parameters associated with pathogenic Acanthamoeba; also, four isolates from swimming pools presented high pathogenic potential. Our results indicate a widespread distribution of potentially pathogenic Acanthamoeba T4, T5, and T2/T6 in different environmental sources in Bras铆lia, revealing the potential risk of human infection and the need of preventive measures.