Molecular interactions of natural and synthetic steroids in female hamsters鈥?flank organs

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• 作者：Marisa Cabeza^a ; ^{marisa@correo.xoc.uam.mx} ; Barak Naranjo^a ; Yvonne Heuze^a ; Araceli Sá ; nchez^a ; Mercedes Herná ; ndez^a ; Teresita Sainz^a ; Eugene Bratoeff^b
• 关键词：Enzymes involved in lipid metabolism ; mRNA expression ; Flank organs ; Levonorgestrel ; Progesterone ; Progesterone derivatives
• 刊名：Journal of Dermatological Science
• 出版年：2012
• 期刊代码：153_09231811
• 类别：med
• 出版时间：May, 2012
• 卷：66
• 期：2
• 页码：119-126
• 文件大小：388 K

摘要

Background

The initial step of steroidal action on target cells is gene activation; therefore, the quantification of mRNA is a direct method for comparing the role of different steroids in the skin.

Objective

This study demonstrated the role of several steroids on the mRNA expression encoding for different enzymes involved in the lipid metabolism in hamsters鈥?flank organs, which are a pilosebaceous complex.

Methods

To determine the effect of treatments with testosterone (T) progesterone (P), levonorgestrel (LNG), 17伪-p-chlorobenzoyloxy-6-chloropregn-4,6-diene-3,20-dione (5) and 17伪-p-chlorobenzoyloxy-4,6-pregnadiene-3,20-dione (6); T and/or LNG; T and 5 or 6; P and/or 5 or 6 on the expression of mRNA encoding for lipid enzymes, the steroids were applied to the glands; later, the mRNAs expression for the enzymes was determined by PCR. The binding of 5 and 6 to the progesterone receptor (PR) was also evaluated.

Results

Treatments with T, LNG, T + LNG, P, T + P, 5, T + 5, T + 6, P, P + 5 and P + 6 increased the mRNA expression for glycerol 3-phosphate acyl transferase (GPAT), 尾-hydroxy-尾-methylglutaryl-CoA synthase (HMG-CoA-S), 尾-hydroxy-尾-methylglutaryl-CoA reductase (HMG-CoA-R), phosphatidylinositol synthase as compared to the controls. However, squalene synthase was increased with all treatments except with T + 5 and 6; 6 did not significantly increase the expression for GPAT or HMG-CoA-S, however it increased the concentration of HMG-CoA-R enzyme.

5 and 6 bind to the PR, thus indicating that the effect of these steroids on the mRNA expression could be the result of their binding.

Conclusion

The lipid metabolism is regulated by several steroids thought different mechanism of action, in flank organs.