The ALCAT test (antigen leukocyte cellular antibody test) is an assay that was developed to assess food intolerance in vitro and is thus used as a diagnostic aid in dietetics and nutrition. The symptoms associated with food intolerance are usually chronic and highly varied and encompass gastrointestinal disorders (such as abdominal pain and bloating), vomiting and diarrhea, dermatitis and eczema, migraine, fluid retention, chronic fatigue, and rheumatic alterations.
The ALCAT test determines cellular reactivity by cytometric reading, which is related to changes in cell number and size. The cell types analyzed by the test (which are the targets of the adverse effects caused by intolerance) are granulocytes, platelets and lymphocytes (the latter manufacture antibodies to food antigens). Thus, the ALCAT test is an assay that reproduces in the laboratory a situation similar to that occurring in the human body when contact is made with food.