A phenolic antioxidant from the Pacific oyster (Crassostrea gigas) inhibits oxidation of cultured human hepatocytes mediated by diphenyl-1-pyrenylphosphine

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• 作者：Mitsugu Watanabe^a ; ^b ; ¹ ; Hirotoshi Fuda^c ; ¹ ; Shigeki Jin^c ; Toshihiro Sakurai^c ; Shu-Ping Hui^c ; Seiji Takeda^c ; Takayuki Watanabe^b ; Takao Koike^a ; Hitoshi Chiba^c ; ^{chibahit@med.hokudai.ac.jp}
• 关键词：AAPH ; 2 ; 2&prime ; -azobis(2-amidinopropane)dihydrochloride ; CCK-8 ; Cell Counting Kit-8 ; DHMBA ; 3 ; 5-dihydroxy-4-methoxybenzyl alcohol ; DPBS ; Dulbecco&rsquo ; s phosphate-buffered saline ; DMSO ; dimethyl sulfoxide ; DPPP ; diphenyl-1-pyrenylphosphine ; EPA-E ; eicos
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 October, 2012
• 卷：134
• 期：4
• 页码：2086-2089
• 文件大小：291 K

摘要

3,5-Dihydroxy-4-methoxybenzyl alcohol (DHMBA), an antioxidant isolated from the Pacific oyster (Crassostrea gigas), was studied in a cell-based fluorometric antioxidant assay using human hepatocyte-derived cells (C3A) and diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe. In comparison with two hydrophilic antioxidants, DHMBA showed the stronger inhibition of DPPP-mediated fluorescence than chlorogenic acid and l-ascorbic acid: at a concentration of 320 渭M of DPPP, the inhibition was 26.4 卤 2.6%, 11.1 卤 1.2%, and 0 卤 2.0%for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean 卤 SD, n = 4). Their relative oxygen radical absorbance capacities (ORAC) were dissociated with their cell-based antioxidant activities: 1.47 卤 0.40, 4.57 卤 0.30, and 0.53 卤 0.13 渭mol TE/渭mol for DHMBA, chlorogenic acid, and l-ascorbic acid, respectively (mean 卤 SD, n = 4). The amphiphilicity of DHMBA was better than chlorogenic acid and l-ascorbic acid might underlie this dissociation. Since the C3A cells are human hepatoma-derived cells, DHMBA might be useful in the prevention and treatment of liver diseases by involving an oxidation process.