Rhophilin-2 is targeted to late-endosomal structures of the vesicular machinery in the presence of activated RhoB

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• 作者：Sé ; verine Steuve ; Thalie Devosse ; Elsa Lauwers ; Jean-Marie V ; erwinden ; Bruno André ; Pierre J. Courtoy ; Isabelle Pirson
• 关键词：Rhophilin ; RhoB ; Multivesicular bodies ; Bro1
• 刊名：Experimental Cell Research
• 出版年：2006
• 期刊代码：292_00144827
• 类别：med
• 出版时间：10 December 2006
• 卷：312
• 期：20
• 页码：3981-3989
• 文件大小：1281 K

摘要

Rhophilin-2 or p76^RBE, a protein whose expression is induced by the cyclic AMP pathway in thyrocytes, contains several protein–protein interaction domains including HR-1, Bro1 and PDZ domains, and is a partner of RhoB in its GTP-bound form (Eur J Biochem, 269(24): 6241–9, 2002). We here define its subcellular localization and dissect the significance of its domains. By subcellular fractionation and colocalization experiments, rhophilin-2 is recruited to subcellular organelles by activated RhoB-GTP. As for its yeast homologue, Npi3/Bro1p, the Bro1 domain of rhophilin-2 is necessary to its recruitment to the vesicular structures, which are not labeled for EEA1 nor Lamp1, but well with the late endosome marker CD63.